Thursday, December 3, 2015

Human DNA extraction lab

In this lab, we asked the question of how can DNA be seperated from cheek cells to study it? Our group found out that DNA was caused by how much saliva we had are from how hard we were rubbing our teeth against our cheek cells. By using saliva formed by our rubbing teeth mixed with gatorade, it percipitated and form our own DNA. We observed that all our own DNA became visible after adding alcohol into the mixture of gatorade, salt( enzyme), and soap detergent, along with our cheek cell's saliva. This is because of lysis since alcohol , along with the enzyme (salt), was able to breaks the cheek cell's semi-permeable membrane. The gatorade was insoluble since with its' many chemicals inside, but it was mainly that was alcohol and process of Protease that broken the proteins of the cheek's cell membrane to extract our DNA. This data supports our claim because chemicals like soap, gatorade, and alcohol can easily be broken from the cheek cell's saliva for people, including scientists to study it.
While our hypothesis was supported by the data we gathered from how DNA was separated from cheek cells, there could have been errors due to the how hard did the students rub their teeth against their cheek cell and how much soap, salt, and gatorade was poured in the small cup. The catabolic of the students' teeth rubbing against the cheek cell and how much drops of soap, pinches of salt, and gatorade poured could have affected when and how the DNA was extracted. For example, our group all saw our DNA extracted after putting all the other liquids once, but did not finish at the same time because of how hard we rubbed our teeth along size of cheek cell. One thing we could have changed was eliminating several materials such as soap detergent, and gatorade, leaving only salt and the catabolic of the cheek cell's saliva. That way, since they are constants of the lab, they won't change(basic as histones) as much and become nonpolar to each other, giving them to be extra careful in how much salt they put and the intensity of their catabolic of teeth rubbing against cheek cells.
This labs done to demonstrate of how chemical reactions can easily extract DNA. With gatorade, salt, and soap, composed with the cheek cell's saliva, it forms our DNA since saliva is where all our bacteria is. This lab helped me understand the concept that the DNA's double helix structure can be broken by any chemical reaction or mutation that changes the letter pairing for the DNA. Based on my experience from this lab, I could apply that to never swallow any chemicals since it can hurt your DNA system.


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