2. The mutations with the greatest change in the DNA genetic code is deletion, then insertion, while the mutations with least change is substitution. By deleting underlined T, the amino- acid sequence changed by the 3rd Amino acid from the original Amino-acid sequence and never was similar to that sequence. Same as inserting a C inside the genetic code instead it started from the 5th amino acid, that was different from the original amino acid sequence. So it does matter where the mutation occur since if the letter T for C, it still did not change the amino- acid sequence until it really started inserting and deleting letters in the DNA sequence.
3. I chose deletion as my mutation because it had the greatest effect on the Amino- acid sequence. While the other mutations had the same amino- acid sequence until the 5th amino acid (Insertion), deletion started to change from the original amino acid sequence in the 3rd amino acid. It does matter of where the mutation occurs since it can possibly harm or give support in your DNA code.
4. Knowing which mutations are harmful or not, helps us by knowing whether to raise more offspring with a mutation. Since there are a variety of mutations, it is not necessary are all harmful or good. A mutation that alter's a human's phenotype, is Progeria which is a mutation in the LMNA gene, which provides supports to cell's nucleus. It causes accelerated aging, along with baldness in body and growth impairment. These people mostly die in when they are 13 years old of heart stroke or attack with rarely some living to their 20s'.
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