Candy Electrophoresis Lab Conclusion

In this lab we explored how by taking the dye out of the candy can be used for gel electrophoresis. When we analyzed our results, we found that only the experimental yellow dye did not match the reference green dye, but all the experimental dyes were a different size to the reference dyes as they were smaller compared to the reference dyes. The structure of Carminic acid migrates similarly to dyes is because like the OH and O sticking out forward, the red and blue dyes are the only dyes to shoot up after gel electrophoresis and the fact that it is a double layered structure, that is why it is Carminic acid.

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For example, a dog food manufactures put artificial food colors(FD&C dyes)  in dog food, so that it prevents the chance of them being sick or having some bad disorder. 2 factors that control the colored solution dyes to migrate is the size and structure of the dye from the candy(M&M, Skittles, or MIke Ikes). These dyes are being moved through the gel by electric voltage so that they are able to spread the dyes out, as shown above. The component of gel electrophoresis that causes the dye's molecules to seperate by size, is when the electric current is present ,dyes spread out by their size, as shown above. Finally, if I had to deal with molecules of 600,1000, 2000, and 5000 daltons, the 600 daltons would separating the furthest, and the 1000 daltons seperating a little further than 2000 daltons, but very close to each other. Then, the 5000 daltons would be still not be seperated its' dye. From this lab, I was able to learn how the dyes in the candies (Skittles, M&Ms', and Mike Ikes) we eat, have different migration patterns under Gel Electrophoresis based, on the size and structure from looking at the candy itself.

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