pGLO lab conclusion

pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB 0(carpet) white light tan - pGLO LB/amp 0 white white + pGLO LB/amp 75 white white + pGLO LB/amp/ara 250 tan/green green

2.	What two new traits do your transformed bacteria have?
	Ampicillin resistance and ability to glow by shining UV light on it.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic. About 1000 microliters of bacteria because adding 25 to each plate and the +Glo LB/amp/ara had 250 colonies which is 10 times the amount of bacteria from adding the bacteria to the plate. so 100(10) equals 1000 microliters.
4.	What is the role of arabinose in the plates?
	To express the gene of the plasmids having that sugar, in order to let bacteria glow.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	It is used to image pathogenic bacteria, an indicator to show when one glows, the other glows, and gives quantitative amount of bacterial association with mammalian cells.
6.	Give an example of another application of genetic engineering. Genetic Engineering helps create Genetically Modified Organisms in Agriculture, and in organisms, determine the functions of their certain genes.