In the Recombinant DNA Lab, me and my partner discovered in order for Recombinant DNA to transfer with another organism’s DNA sequence, we needed to identify the restriction enzymes, specifically those that are closer to the insulin gene. If bacteria takes in our plasmid, I would chose antibiotics of ampicillin, and kanamycin, because ampicillin gives treatment probably too the most important systems of the body, urinary, that is used to pee and respiratory system to breath normally,and kanamycin is an antibiotic drawn from a strand of bacteria, so I would want an antibiotic that is used to fighting against bacteria, because it will know what to do if bacteria enters the immune system I would not use tetracycline, because it gives treatment to those who have four legs, and humans have two legs. Restriction enzymes cut DNA fragments and have a “sticky end” that allows DNA to recombine with each other.I chose Eco RI, mainly as my restriction enzyme since it was the closest to the insulin gene I found in the red enzyme. If we cut plasmids, we would have to find 2 different organisms that can use ligase to connect restriction enzymes from DNA sequences together. This relates to our daily life of whenever we get sick, we use antibiotics to resist the plasmids in the bacteria, in order to maintain our DNA sequence in order, and healthy. This Recombinant DNA process can be used to determine the genetic disorders in someone’s DNA sequence, and what antibiotics or medicine should they take(gene therapy; Medical and Pharmaceutical Biotech).
Couldn’t take any photos,due to class time. Sorry.
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